2018
Lors de la 3ème édition du congrès “Analytical methods to study oxidative damages, antioxidants and drugs” qui se déroulera du 24 au 26 Mai 2018 à Bialystok (Pologne), le Dr Caroline GAUCHER donnera une conférence intitulée "Protein S-nitrosation as a biomarker of nitric oxide bioavailability under oxidative stress"
L'article "Glutathione: antioxidant properties dedicated to nanotechnologies" vient de paraître dans le journal "Antioxidants", special issue "Inspired by Nature: Antioxidants and Nanotechnologies".
Auteurs : Gaucher C., Boudier A., Bonetti J., Clarot I., Leroy P., Parent M.
Référence de l'article : Antioxidants 2018, 7(5), 62; https://doi.org/10.3390/antiox7050062
Abstract :
Which scientist has never heard of glutathione (GSH)? This well-known low-molecular-weight tripeptide is perhaps the most famous natural antioxidant. However, the interest in GSH should not be restricted to its redox properties. This multidisciplinary review aims to bring out some lesser-known aspects of GSH, for example, as an emerging tool in nanotechnologies to achieve targeted drug delivery. After recalling the biochemistry of GSH, including its metabolism pathways and redox properties, its involvement in cellular redox homeostasis and signaling is described. Analytical methods for the dosage and localization of GSH or glutathiolated proteins are also covered. Finally, the various therapeutic strategies to replenish GSH stocks are discussed, in parallel with its use as an addressing molecule in drug delivery.
Communication orale au congrès Printemps de la Cardiologie :
S-nitrosation decreases AT1 receptor mediated vasoconstriction and myogenic tone on rat middle cerebral arteries par M-L BOURESSAM, Doctorante
Présentation orale lors de la Journée Scientifique de l'école doctorale BioSE du 28 mars 2018 :
Derivatization methods with liquid chromatography- HCD tandem mass spectrometry for the measurement of nitric oxide species coming from S-nitrosoglutathione par H. YU, Doctorante
L'article "Polymeric nanoparticles for increasing oral bioavailability of curcumin" vient de paraître dans le journal "Antioxidants", dans l'issue spéciale "Inspired by nature : Antioxidants and Nanotechnology".
Auteurs : Anita Umerska, Caroline Gaucher, Felipe Oyarzun-Ampuero, Isabelle Fries-Raeth, Florence Colin, Maria Gabriela Villamizar-Sarmiento, Philippe Maincent, Anne Sapin-Minet.
Référence de l'article : Antioxidants 2018, 7(4), 46; https://doi.org/10.3390/antiox7040046
Article accessible en cliquant ici.
Abstract :
Despite the promising biological and antioxidant properties of curcumin, its medical applications are limited due to poor solubility in water and low bioavailability. Polymeric nanoparticles (NPs) adapted to oral delivery may overcome these drawbacks. Properties such as particle size, zeta potential, morphology and encapsulation efficiency were assessed. Then, the possibility of storing these NPs in a solid-state form obtained by freeze-drying, in vitro curcumin dissolution and cytocompatibility towards intestinal cells were evaluated. Curcumin-loaded Eudragit® RLPO (ERL) NPs showed smaller particle diameters (245 ± 2 nm) and better redispersibility after freeze-drying than either poly(lactic-co-glycolic acid) (PLGA) or polycaprolactone (PCL) NPs. The former NPs showed lower curcumin encapsulation efficiency (62%) than either PLGA or PCL NPs (90% and 99%, respectively). Nevertheless, ERL NPs showed rapid curcumin release with 91 ± 5% released over 1 h. The three curcumin-loaded NPs proposed in this work were also compatible with intestinal cells. Overall, ERL NPs are the most promising vehicles for increasing the oral bioavailability of curcumin.
Offres de recrutements (un doctorat et un post-doctorat) financés par Lorraine Université d'Excellence (LUE) IMPACT Biomolecules. Date limite d'envoi de candidature : 30 avril 2018.
Pour plus de renseignements, contacter le Dr Caroline GAUCHER caroline.gaucher@univ-lorraine.fr
Lors de la 2ème réunion du COST Action NutRedOx qui se déroule du 15 au 16 février 2018 à Palma de Majorque en Espagne, Le Dr Caroline GAUCHER donnera une conférence sur le projet "Impact Biomolécules" financé par Lorraine Université d'Excellence (UL).
nanoCONTROL, une Start-up issue des travaux de l'EA 3452.
Les travaux de l'EA sur les nanoparticules d'or ont mené, avec le soutien de l'incubateur lorrain, à l'incubation d'une Start-up depuis le 05 décembre 2017.
Ce projet intitulé "nanoCONTROL" porte sur le contrôle et la synthèse de nanoparticules de très grande qualité "grade pharmaceutique".
L'article "Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles" vient de paraître dans le journal "Talanta"
Auteurs : Pallotta A., Philippe V., Boudier A., Leroy P., Clarot I.
Référence de l'article : Talanta. 179 (2018) 307-311 Doi : 10.1016/j.talanta.2017.11.016
Abstract :
A simple isocratic HPLC method using visible detection was developed and validated for the quantification of gold in nanoparticles (AuNP). After a first step of oxidation of nanoparticles, an ion-pair between tetrachloroaurate anion and the cationic dye Rhodamine B was formed and extracted from the aqueous media with the help of an organic solvent. The corresponding Rhodamine B was finally quantified by reversed phase liquid chromatography using a Nucleosil C18 (150mm × 4.6mm, 3µm) column and with a mobile phase containing acetonitrile and 0.1% trifluoroacetic acid aqueous solution (25/75, V/V) at 1.0mLmin-1.and at a wavelength of 555nm. The method was validated using methodology described by the International Conference on Harmonization and was shown to be specific, precise (RSD < 11%), accurate and linear in the range of 0.1 - 30.0µM with a lower limit of quantification (LLOQ) of 0.1µM. This method was in a first time applied to AuNP quality control after their synthesis. In a second time, the absence of gold leakage (either as AuNP or gold salt form) from nanostructured multilayered polyelectrolyte films under shear stress was assessed.