Nous sommes heureux d’annoncer la nomination à l’Institut Universitaire de France comme membre Junior en chaire innovation de Ariane Boudier.
C’est une excellente nouvelle !
Nous sommes heureux d’annoncer la nomination à l’Institut Universitaire de France comme membre Junior en chaire innovation de Ariane Boudier.
C’est une excellente nouvelle !
Un article intitulé " Activity and reusability of immobilized gold nanoparticles for the catalysis of both oxidation and reduction reactions " vient de paraître dans "Results in Chemistry"
Auteurs : Boukoufi C, Boudier A, Lahouari S, Vigneron J, Clarot I
Results in Chemistry, in press, Journal pre-proof, 100979, https://doi.org/10.1016/j.rechem.2023.100979
Abstract
Colloidal gold nanoparticles (AuNP) are well-known to present a catalytic activity. However, they are characterized by certain limitations, as a rather poor stability and lack of both handling and reusability. Their immobilization on surfaces could help to overcome these drawbacks. This work evaluated the impact of the immobilization of AuNP towards their catalytic activity on reduction and oxidation reactions. Colloidal AuNP stabilized with citrate ions were synthesized, characterized, and immobilized by the dip-coating method, the obtained immobilized AuNP (iAuNP) were characterized. The catalytic activity of AuNP and iAuNP was evaluated and compared to each other through the p-nitrophenol reduction reaction. The kinetic of the catalytic activity of iAuNP was slower than colloidal AuNP. The catalytic activity appeared to be impacted by the less available surface of iAuNP. The reduction efficiency of iAuNP was also evaluated with the DPPH•/DPPH2 reduction reaction. The capacity of iAuNP to catalyze the reduction of DPPH• in DPPH2 was shown even after 130 days and 20 reuses of the nanostructured surface on the contrary to non-reusable colloidal AuNP. The oxidation efficiency of iAuNP was measured by the ability to catalyze the auto-oxidation of curcumin (HPLC-UV/vis). For all these experiments, the addition of 4-mercapto-phenol on the surface of iAuNP passivated the surface, decreasing the catalytic activity of the materials. In conclusion, iAuNP are redox catalysts with both anti- and pro-oxidant effects and high reusability over a very long period.
Un article intitulé "Cross-frontal mode: An alternative methodology for Taylor dispersion analysis of monomodal sample" vient de paraître dans "Journal of Chromatography A".
Auteurs : Gouyon J, Boudier A, Pallotta A, Boukoufi C, Clarot I
Journal of Chromatography A, Volume 1694, 2023, 463913.https://doi.org/10.1016/j.chroma.2023.463913
Abstract
Taylor dispersion analysis (TDA) is a technique dedicated to the determination of the molecular diffusion coefficient (D) of species, using band broadening of an analyte in a laminar flow. Two modes are commonly used to perform TDA: pulse and frontal modes. In each case, a fitting of the signal is required. We propose here a third mode denoted as cross-frontal mode, combining two crossed sample fronts without modification of a classical CE device for the rapid and accurate determination of D of caffeine, reduced glutathione (GSH), insulin from bovine pancreas, bovine serum albumin (BSA) and citrate-capped gold nanoparticles (AuNP). Theoretical aspects and methodology are described, showing a good correlation between the so-called cross-frontal mode and usual frontal mode. Limitations of the techniques are also assessed, and are similar to regular modes while no fitting is required. This new methodology allows improving the sensitivity toward low concentrated sample compared to pulse mode, and an alternative mathematical treatment compared to regular TDA modes.
Un article intitulé " Metal-chelating activity of soy and pea protein hydrolysates obtained after different enzymatic treatments from protein isolates " vient de paraître dans " Food Chemistry "
Auteurs : Sarah El Hajj, Rachel Irankunda, Jairo Andrés Camaño Echavarría, Philippe Arnoux, Cédric Paris, Loic Stefan, Caroline Gaucher, Sandrine Boschi-Muller, Laetitia Canabady-Rochelle
Food Chemistry, Volume 405, Part A, 134788. https://doi.org/10.1016/j.foodchem.2022.134788
Abstract
Soy and pea proteins are two rich sources of essential amino acids. The hydrolysis of these proteins reveals functional and bioactive properties of the produced small peptide mixtures. In our study, we employed the hydrolysis of soy and pea protein isolates with the endopeptidases Alcalase® and Protamex®, used alone or followed by the exopeptidase Flavourzyme®. The sequential enzyme treatments were the most efficient regarding the degree of hydrolysis. Then, soy and pea protein hydrolysates (SPHs and PPHs, respectively) were ultrafiltrated in order to select peptides of molecular weight ≤ 1 kDa. Whatever the protein source or the hydrolysis treatment, the hydrolysates showed similar molecular weight distributions and amino acid compositions. In addition, all the ultrafiltrated hydrolysates possess metal-chelating activities, as determined by UV-spectrophotometry and Surface Plasmon Resonance (SPR). However, the SPR data revealed better chelating affinities in SPHs and PPHs when produced by sequential enzymatic treatment.
Un article intitulé " Taylor dispersion analysis of metallic-based nanoparticles – A short review " vient de paraître dans " Electrophoresis "
Auteurs : Jeremie Gouyon, Ariane Boudier, Fatima Barakat, Arnaud Pallotta, Igor Clarot
Electrophoresis, 2022, https://doi.org/10.1002/elps.202200184
Abstract
Taylor dispersion analysis (TDA) is an interesting tool for nanoparticle (NP) size determination, feasible using simple capillary electrophoresis apparatus. Based upon the radial diffusion of analytes upon a laminar stream, the diffusion coefficient of species is easily estimable. Moreover, TDA is generally more adequate than conventional dynamic light scattering methodologies as it is less dependent on the polydispersity of the sample, leading to accurate measurement and reliable results. This review provides every paper mentioning the use of TDA for metallic-based NPs size determination. Diverse strategies for the detection of metallic NPs (like UV–visible and inductively coupled plasma–mass spectrometry – ICP–MS – for instance) and interpretation of the Taylorgrams are discussed. Based upon the literature, advices on future prospects are also indicated, especially for the comparison of TDA results with other classical techniques.
Un article intitulé " Thiol sensing: From current methods to nanoscale contribution " vient de paraître dans " Microchemical Journal "
Auteurs : Margaux Berthou, Igor Clarot, Jeremie Gouyon, Damien Steyer, Marie Anais Monat, Ariane Boudier, Arnaud Pallotta
Microchemical Journal, 183, 2022, 107994. https://doi.org/10.1016/j.microc.2022.107994
Abstract
Thiols play critical roles in many biologicals processes: they are involved in combating oxidative stress and maintaining redox homeostasis. In agri-food, they are responsible for organoleptic aspect of many foodstuffs. Thus, thiol identification and quantification are challenging due to their similar chemical structures (especially for small/volatile thiols), reactivity and matrices of these compounds. To overcome the analytical issues, many methods and technics were developed each with their own advantages and drawbacks. Lately, one emerging field in analytical development goes through the contribution of nanoparticles. Scientific teams rely on their unique properties to improve sensitivity, selectivity and decrease matrices effects. This review is an overview of current methods for thiol detection followed by the report of nanomaterial contribution to the field. Finally, a critical point of view will be given to these new sensing methods, from their potential to their limits.
Margaux BERTHOU soutiendra sa thèse de doctorat intitulée "Monitoring de thiols et S-nitrosothiols à l'aide d'un dispositif nanostructuré" le mardi 20 septembre à 9h dans l'amphithéatre Lepois du campus Brabois santé.
Le jury est composé de :
- Mme Catherine FOULON (Rapporteur, Pr, EA 7365, Université de Lille, Lille)
- Mme Murielle ROCHELET (Rapporteur, Dr, AgroSup, Université de Bourgogne)
- Mme Corine RAVELET (Examinateur, Pr, UMR 5063, Université de Genoble-Rhone-Alpes, Grenoble)
- M. Emmanuel LAMOUROUX (Examinateur, Dr, UMR 7565 Université de Lorraine, Nancy)
- M. Igor CLAROT (Examinateur, PR, EA 3452 CITHEFOR Université de Lorraine, Nancy, Directeur de thèse)
- M. Arnaud PALLOTTA (Examinateur, Dr, EA 3452 CITHEFOR Université de Lorraine, Nancy, Co-directeur de thèse)
Nous accueillons au sein de l'équipe de nouveaux arrivants :
- Franceline Reynaud, Maitre de Conférences en Pharmacie galénique
- Gladys Bantsimba Malanda, ATER en Pharmacologie
- Diego Ramos, ATER en Pharmacie Galénique
Bienvenue à eux trois !
Un article intitulé " Succinimido–Ferrocidiphenol Complexed with Cyclodextrins Inhibits Glioblastoma Tumor Growth In Vitro and In Vivo without Noticeable Adverse Toxicity " vient de paraître dans " Molecules "
Auteurs : Feten Najlaoui, Benoit Busser, Germain Sotoing Taïwe, Pascal Pigeon, Nathalie Sturm, Diane Giovannini, Naziha Marrakchi, Ali Rhouma, Gérard Jaouen, Stéphane Gibaud et Michel De Waard
Molecules 2022, 27(14), 4651; https://doi.org/10.3390/molecules27144651
Abstract
SuccFerr(N-[4-ferrocenyl,5-5-bis(4-hydroxyphenyl)-pent-4-enyl]-succinimide) has remarkable antiproliferative effects in vitro, attributed to the formation of a stabilized quinone methide. The present article reports in vivo results for a possible preclinical study. SuccFerr is lipophilic and insoluble in water, so the development of a formulation to obviate this inconvenience was necessary. This was achieved by complexation with randomly methylated cyclodextrins (RAMEßCDs). This supramolecular water-soluble system allowed the in vivo experiments below to proceed. Application of SuccFerr on the glioblastoma cancer cell line U87 indicates that it affects the cellular cycle by inducing a blockade at G0/G1 phase, linked to apoptosis, and another one at the S phase, associated with senescence. Using healthy Fischer rats, we show that both intravenous and subcutaneous SuccFerr: RAMEßCD administration at 5 mg/kg lacks toxic effects on several organs. To reach lethality, doses higher than 200 mg/kg need to be administered. These results prompted us to perform an ectopic in vivo study at 1 mg/kg i.v. ferrocidiphenol SuccFerr using F98 cells xenografted in rats. Halting of cancer progression was observed after six days of injection, associated with an immunological defense response linked to the active principle. These results demonstrate that the properties of the selected ferrocidiphenol SuccFerr transfer successfully to in vivo conditions, leading to interesting therapeutic perspectives based on this chemistry.
La maladie de Menkès est une pathologie rare d’origine génétique qui induit un déficit en cuivre fatal pour les enfants (décès avant l’âge de trois ans). Malheureusement, il n’existe à l’heure actuelle aucun traitement médicamenteux disponible sur le marché. Deux laboratoires de l’UL (le NGERE et le CITHEFOR) développent depuis 4 ans un transporteur synthétique capable de véhiculer le cuivre jusqu’au cerveau. Le nanotransporteur synthétisé est stable, biocompatible, non toxique et efficace. Une procédure réglementaire accélérée est actuellement en discussion avec l’Agence Européenne des Médicaments et le consortium espère une demande d’autorisation de mise sur le marché pour 2023.
Plus d'infos dans l'article de Factuel